Journal: Diseases of aquatic organisms
Article Title: Antibody response of channel catfish after channel catfish virus infection and following dexamethasone treatment.
doi: 10.3354/dao02348
Figure Lengend Snippet: Fig. 2. Western blot analysis of mock- and channel catfish virus (CCV)-infected brown bullhead (BB) cell lysates using catfish serum. Cell lysates and supernatants were separated by 12% SDS-PAGE and blotted onto nitrocellulose mem- branes. (A) Positive control catfish serum against mock- infected cell lysates (L1) and supernatants (S1) or aganist CCV-infected cell lysates (L2) and supernatants (S2). Markers are Bio-Rad Prestained SDS-PAGE broad range molecular weight markers (M1) and Amersham ECL protein molecular weight markers (M2). (B) The humoral immune profile at 0, 30, 60, and 90 d post challenge, and 10 d post treatment (PT) with dexamethasone (DEX, 0.55 mg kg–1 body weight) from 2 CCV/DEX-treated fish (lanes 1 to 5 and 6 to 10) representing positive samples and from 1 mock/DEX-treated fish (lanes 11 to 15) representing a negative sample in western blots. Posi- tive (+) and negative (–) fish serum controls; M = ECL protein molecular weight markers (Amersham). No bands were detected with these samples in blots on mock-infected BB cell lysates (patterns were identical to negative fish serum control)
Article Snippet: Markers are Bio-Rad Prestained SDS-PAGE broad range molecular weight markers (M1) and Amersham ECL protein molecular weight markers (M2). (B) The humoral immune profile at 0, 30, 60, and 90 d post challenge, and 10 d post treatment (PT) with dexamethasone (DEX, 0.55 mg kg–1 body weight) from 2 CCV/DEX-treated fish (lanes 1 to 5 and 6 to 10) representing positive samples and from 1 mock/DEX-treated fish (lanes 11 to 15) representing a negative sample in western blots.
Techniques: Western Blot, Virus, Infection, SDS Page, Positive Control, Molecular Weight, Control